Bacteria in endodontics

Apical Periodontitis

16S rDNA PCR-DGGE approach

Band No. Bacterial species Similarity with nearest matches (%) GenBank accession number Total (% of isolates)
1 P. endodontalis 98 AB547659.1 4 (30.8)
2 Capnocytophaga gingivalis 100 AB638448.1 2 (15.4)
3 Pseudoramibacter alactolyticus 100 AB036761.1 3 (23.1)
4 Firmicutes sp. oral clone CK057 100 AF287778.1 1 (7.7)
5 Dialister invisus clone WWP_SS10_G28 99 GU401628.1 3 (23.1)
6 Bilophila wadsworthia 99 L35148.1 2 (15.4)
7 Uncultured bacterium clone ncd2794c06c1 100 JF239946.1 5 (38.5)
8 Propionibacter sp. oral clone HE018 100 DQ087192.1 8 (61.5)
9 Propionibacterium propionicus 98 AF285117.1 6 (46.2)
10 Actinomyces sp. oral clone EL030 100 AY008311.1 11 (84.6)
11 Streptococcus mitis strain C91 99 JF803497.1 5 (38.5)
12 Atopobium sp. F0209 99 EU592966.1 2 (15.4)
13 Burkholderia sp. Rc07 99 EU438869.1 4 (30.8)
14 Uncultured beta proteobacterium clone A12-72 99 JN371580.1 3 (23.1)
15 Uncultured delta proteobacterium clone D009011D04 100 GU179673.1 3 (23.1)
16 Neisseria sp. oral clone AP085 99 AY005023.1 2 (15.4)
17 Selenomonas sp. oral taxon 149 clone WWP_SS5_C24 100 GU403271.1 3 (23.1)
18 Prevotella oralis ATCC 33269 99 NR_042841.1 2 (15.4)
19 Uncultured Spirochaeta sp. clone RP9-182 16S 99 HM049870.1 2 (15.4)
20 Prevotella denticola 100 AB547679.1 3 (23.1)
21 Prevotella nigrescens 99 AB547696.1 2 (15.4)
22 Uncultured bacterium clone ncd2814f09c1 JF241249.1 4 (30.8)
23 Prevotella sp. oral clone IDR-CEC-0032 99 AY550995.1 2 (15.4)
24 Veillonellaceae bacterium oral taxon 131 clone WWP_SS10_C24 99 GU402914.1 1 (7.7)
25 Neisseria bacilliformis ATCC BAA-1200 99 NR_042978.1 3 (23.1)
26 Bifidobacterium dentium strain KCTC 3222 97 GU361819.1 2 (15.4)

Intra-pulpal origin

Exposed v unexposed the species are very similar but quantity is higher in unexposed.